Streptozocin
作者：齊岳
發(fā)布時(shí)間：2023-09-11
09:05:32
點(diǎn)擊數(shù)：
產(chǎn)品名稱：Streptozocin
產(chǎn)品描述：
| 植物來(lái)源 | 天然產(chǎn)物 > 其他（植物來(lái)源） > 其他 |
| 產(chǎn)品描述 | Streptozotocin is an antibiotic that is produced by Stretomyces achromogenes. It is used as an antineoplastic agent and to induce diabetes in experimental animals. |
| 靶點(diǎn)活性 | DNA-methylating (K562 cell):904 μg/mL, DNA-methylating (HL60 cell):11.7 μg/mL, DNA-methylating (C1498 cell):1024 μg/mL |
| 體外活性 | Streptozotocin通過低親和力的GLUT 2葡萄糖轉(zhuǎn)運(yùn)體在胰島β細(xì)胞中選擇性積累.注射4個(gè)月60 mg/kg Streptozotocin會(huì)誘導(dǎo)β細(xì)胞快速脫粒而不壞死,發(fā)生白內(nèi)障并且在腎的近曲小管中積累糖原.在患有' Streptozotocin 糖尿病'大鼠體內(nèi),100 mg/kg Streptozotocin損傷胰腺外分泌細(xì)胞,使小的可能分泌的顆粒在β細(xì)胞的高爾基體中持續(xù)存在.Streptozotocin在大鼠、小鼠和倉(cāng)鼠體內(nèi)被發(fā)現(xiàn)是致*的. |
| 體內(nèi)活性 | Streptozotocin對(duì)胰島β細(xì)胞具有毒性。Streptozotocin直接甲基化DNA并具有高度遺傳毒性,產(chǎn)生DNA鏈斷裂,堿不穩(wěn)定位點(diǎn),非計(jì)劃DNA合成,DNA加合物,染色體畸變,微核,姊妹染色單體交換和細(xì)胞死亡。暴露于15 mM Streptozotocin1小時(shí),然后24小時(shí)恢復(fù)期誘導(dǎo)鼠胰腺β細(xì)胞系INS-1的細(xì)胞凋亡。30 mM Streptozotocin引起22%的細(xì)胞壞死及17%的細(xì)胞凋亡。 |
| 細(xì)胞實(shí)驗(yàn) | Streptozocin (STZ) stock solutions of 50 mg/mL in distilled Water (dWater) are freshly prepared for each experiment[2]. Human and murine cell lines are cultured in triplicate in 96-well plates at a density of 2×104 cells/well in the absence (untreated control) or presence of various concentrations of ALX (20-3000 μg/mL) or STZ (1-3000 μg/mL) for 48 h at 37°C under a humidified atmosphere containing 5% CO2. Cells incubated in complete media including dWater in a final concentration of 0.1% served as control for solvent toxicity and cells incubated in complete medium are used as a control for the experiments. The effects of the tested drugs on tumor cell growth or viability are determined employing the MTT assay in accordance with the manufacturer's instructions. The IC50values (drug concentration that induces 50% inhibition of the cell growth) are calculated using the GraphPad Prism 4 program[2]. |
| 別名 | STZ, NSC-85998, 鏈脲佐菌素, 鏈脲菌素, U 9889, Streptozotocin |
| 分子量 | 265.22 |
| 分子式 | C8H15N3O7 |
| CAS No. | 18883-66-4 |
存儲(chǔ)
Powder: -20°C for 3 years | In solvent: -80°C for 2 years
溶解度
H2O: 113.3 mg/mL (427.19 mM),Need ultrasonic and warming
DMSO: 30 mg/mL (113.11 mM)
( < 1 mg/mL refers to the product slightly soluble or insoluble )
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