Gimeracil
作者：齊岳
發(fā)布時(shí)間：2023-09-08
09:03:36
點(diǎn)擊數(shù)：
產(chǎn)品名稱：Gimeracil
產(chǎn)品描述：
| 產(chǎn)品描述 | Gimeracil is a competitive, reversible inhibitor of dihydropyrimidine dehydrogenase. |
| 激酶實(shí)驗(yàn) | Microdilution method: The culture media used are RPMI 1640 with glutamine, without bicarbonate and phenol red, buffered with morpholinopropanesulfonic acid (MOPS) (0.165 M, pH 7.0). Two-fold serial dilutions of Flucytosine (0.06-64 μg/mL) are prepared and dispensed in 50 uL aliquot, in flat-bottom 96-well assay plates which are kept frozen at -70 °C in sealed plastic bags until used. The inoculum is prepared spectrophotometrically and standardized to a concentration of 1.0-5.0 × 103 cfu per mL. A 50 μL volume of this suspension is used to inoculate each well containing 50 μL of the double concentration of Flucytosine to be tested. Once inoculated, each well therefore contains 100 μL of broth favoured over 200 μL to facilitate the agitation of the plates prior to spectrophotometric reading. After an incubation period of 24 and 48 hours at 35 °C, the plates are agitated for 3 minutes at 900 r.p.m. with a shaker and the optical density of the growth in each well is determined with the use of an automatic plate reader set at 495 nm. The inhibitory concentration of IC50 is computed mathematically. |
| 別名 | 吉美嘧啶, 吉莫斯特, Gimestat |
| 分子量 | 145.54 |
| 分子式 | C5H4ClNO2 |
| CAS No. | 103766-25-2 |
存儲
Powder: -20°C for 3 years | In solvent: -80°C for 2 years
溶解度
Ethanol: <1 mg/mL
H2O: <1 mg/mL
DMSO: 28 mg/mL (192.4 mM)
( < 1 mg/mL refers to the product slightly soluble or insoluble )
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